rHu EGF Protein

Product Summary

• High purity human EGF protein (residues 971-1023, Uniprot: P01133)
• 6.3 kDa
• >98%, by SDS-PAGE quantitative densitometry
• Expressed in E. coli
• Animal origin-free (AOF) and carrier protein-free
• Manufactured in Cambridge, UK at Qkine laboratories
• Lyophilized from acetonitrile, TFA

Alternative Names

• Epidermal growth factor, Pro-epidermal growth factor, HOMG4, Beta-urogastrone, Urogastrone (URG), Qk11

Reconstitution

Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)

For Use Statement

For research use only. This product is not for human use or for direct clinical use as a drug, therapeutic, biologic or medical device.

Certificate of Analysis (CoA)
Certificate of Origin (CoO)
Safety Data Sheet (SDS)
Product Page

Members of the human epidermal growth factor family are synthesized as type I transmembrane precursor proteins, often containing several EGF domains in the extracellular region. Proteolysis yields mature proteins that are released from the cell surface [1]. Epidermal growth factor binds to high-affinity EGF receptors (EGFRs) and promotes receptor dimerization and clustering leading to activation of downstream signaling pathways, including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling [2].

Human epidermal growth factor (EGF) stimulates cell proliferation and differentiation. It is used extensively in induced-pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture systems for the successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages [3]. EGF also stimulates the differentiation of mesenchymal stem cells (MSC) into bone-forming cells [4].

Recombinant EGF protein is a key component of many organoid media, along with other stem cell niche factors, R-Spondin 1, noggin or gremlin, Wnt3a and FGF-10. In addition, recombinant EGF is used for establishing and maintaining intestine, stomach, liver, pancreas, brain and cancer organoids [5].

[1] R. C. Harris, E. Chung and R.J. Coffey. EGF receptor ligands. Experimental cell research 284, 2–13 (2003). doi.org/10.1093/nar/14.21.8427

[2] G. Carpenter and S. Cohen. Epidermal growth factor. Journal of biological chemistry 265, 7709–7712 (1990). doi.org/10.1016/S0021-9258(19)38983-5

[3] A. G. Efthymiou, G. Chen, M. Rao, G. Chen and M. Boehm, M. Self-renewal and cell lineage differentiation strategies in human embryonic stem cells and induced pluripotent stem cells. Expert Opinion on Biological Therapy, 14(9), 1333–1344 (2014). doi.org/10.1517/14712598.2014.922533

[4] I. Kratchmarova, B. Blagoev, M. Haack-Sorensen, M. Kassem and M. Mann. Mechanism of Divergent Growth Factor Effects in Mesenchymal Stem Cell Differentiation. Science 308,1472-1477 (2005). doi:10.1126/science.1107627

[5] H. E. Abud, W. H. Chan and T. Jardé. Source and Impact of the EGF Family of Ligands on Intestinal Stem Cells. Front Cell Dev Biol. 2021 Jul 19;9:685665. doi: 10.3389/fcell.2021.685665