rHu IL-3 Protein

Product Summary:

• Highly pure human protein (UniProt: P08700)
• 15.2 kDa (monomer)
• >98%, by SDS-PAGE quantitative densitometry
• Expressed in E. coli
• Animal origin-free (AOF) and carrier protein-free
• Manufactured in Cambridge, UK at Qkine laboratories
• Lyophilized from acetonitrile, TFA
  

Alternative Names:

• Multilineage colony-stimulating factor; Hematopoietic growth factor; P-cell stimulating factor; Mast-cell growth factor; Colony-stimulating factor, IL 3, IL3, Qk90

Reconstitution:

Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)

For Use Statement:

For research use only. This product is not for human use or for direct clinical use as a drug, therapeutic, biologic or medical device.

Certificate of Analysis (CoA)
Certificate of Origin (CoO)
Safety Data Sheet (SDS)
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Interleukin 3 (IL-3) also known as B-cell stimulatory factor 2/interferon beta 2, is a pleiotropic cytokine with a crucial role in hematopoiesis and multi-lineage growth factor [1–4]. It promotes the proliferation, differentiation, and survival of hematopoietic stem cells and myeloid progenitors [3–6]. IL-3 induces the development of mast cells, basophils, monocytes, erythrocytes, and megakaryocytes and mediates the release of mediators from these cells. It is also involved in the recruitment of myeloid cells to inflammation sites and regulating T-cell function [2].

[1] Ikebuchi, K. et al. Interleukin 6 enhancement of interleukin 3-dependent proliferation of multipotential hemopoietic progenitors. Proc. Natl. Acad. Sci. 84, 9035–9039 (1987).

[2] Ihle, J. N. Interleukin-3 and hematopoiesis. Chem. Immunol. 51, 65–106 (1992).

[3] Schrader, J. W. Interleukin-3. in Growth Factors and Cytokines in Health and Disease (eds. Leroith, D. & Bondy, C.) vol. 2 49–84 (JAI, 1997).

[4] Pixley, F. J. & Stanley, E. R. Chapter 319 - Cytokines and Cytokine Receptors Regulating Cell Survival, Proliferation, and Differentiation in Hematopoiesis. in Handbook of Cell Signaling (Second Edition) (eds. Bradshaw, R. A. & Dennis, E. A.) 2733–2742 (Academic Press, 2010). doi:10.1016/B978-0-12-374145-5.00319-3.

[5] Broughton, S. E. et al. The GM–CSF/IL-3/IL-5 cytokine receptor family: from ligand recognition to initiation of signaling. Immunol. Rev. 250, 277–302 (2012).

[6] Kölle, J. et al. Targeted deletion of Interleukin-3 results in asthma exacerbations. iScience 25, 104440 (2022).