rHu PDGF BB (Yeast)
Cat#
Size
Store/Ship
AK8274-0002
2µg
2-8° C (-20 LT)
AK8274-0010
10µg
2-8° C (-20 LT)
AK8274-1000
1mg
2-8° C (-20 LT)
Bulk pricing available upon request.
To place an order, please contact our sales team at:
E sales@akronbiotech.com • T 561.750.6120 • T-Free 1.888.702.5766
To place an order, please contact our sales team at:
E sales@akronbiotech.com • T 561.750.6120 • T-Free 1.888.702.5766
Description
Recombinant Human Platelet Derived Growth Factor-BB (Yeast)
Description: Recombinant Human PDGF-BB, produced in yeast, is a double, glycosylated polypeptide chain containing 109 amino acids with a molecular mass of approximately 32 Kda.
Source: A DNA sequence encoding the mature human PDGF-B chain protein sequence was obtained from platelet mRNA, sub cloned into an expression vector and expressed in yeast.
First five aminoacids: Ser-Leu-Gly-Ser-Leu
Physical State of the Product: Sterile filtered white lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 100 mM Acetic Acid solution, without additives.
Solubility: It is recommended to reconstitute the lyophilized PDGF-BB in sterile, distilled water to 0.1 - 1.0 mg/ml, which can then be further diluted to other aqueous solutions.
Purity: greater than 97% as determined by:
-Analysis by reducing and non-reducing SDS-PAGE Silver Stained
Protein Content: Each vial contains 1 mg lyophilized PDGF-BB.
Protein quantitation was carried out by two independent methods:
-Protein assay Quanti-iT Protein Assay Kit for the Qubit fluoremeter (Invitrogen)
-Bradford assay for protein quantification
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis and Western Blot.
Storage: Store lyophilized PDGF-BB desiccated at 2°-8º C. Following reconstitution, store aliquots at -20º C.
Stability: Lyophilized Platelet-derived Growth Factor BB is stable for at least a year. Upon reconstitution, the preparation is stable for up to 10 days at 2-8º C and greater than 3 month at -20º C. Avoid repeated freeze-thaw cycles.
Molecular Mass: 32 KDa.
Biological Activity: Recombinant Human PDGF-BB is fully biologically active when compared to the NIBSC International Standard (IS) for platelet-derived growth factor-BB, which has a specific activity of 3000 I.U/4ug. The ED50, calculated in a dose-dependant proliferation of Swiss mouse 3T3 fibroblasts assay (measured by 3H-thymidine uptake) is 1-3 ng/ml.
Customized sizes upon request.
Usage: For research use only. Not for use in human diagnostics or therapeutic procedures.
Description: Recombinant Human PDGF-BB, produced in yeast, is a double, glycosylated polypeptide chain containing 109 amino acids with a molecular mass of approximately 32 Kda.
Source: A DNA sequence encoding the mature human PDGF-B chain protein sequence was obtained from platelet mRNA, sub cloned into an expression vector and expressed in yeast.
First five aminoacids: Ser-Leu-Gly-Ser-Leu
Physical State of the Product: Sterile filtered white lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 100 mM Acetic Acid solution, without additives.
Solubility: It is recommended to reconstitute the lyophilized PDGF-BB in sterile, distilled water to 0.1 - 1.0 mg/ml, which can then be further diluted to other aqueous solutions.
Purity: greater than 97% as determined by:
-Analysis by reducing and non-reducing SDS-PAGE Silver Stained
Protein Content: Each vial contains 1 mg lyophilized PDGF-BB.
Protein quantitation was carried out by two independent methods:
-Protein assay Quanti-iT Protein Assay Kit for the Qubit fluoremeter (Invitrogen)
-Bradford assay for protein quantification
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis and Western Blot.
Storage: Store lyophilized PDGF-BB desiccated at 2°-8º C. Following reconstitution, store aliquots at -20º C.
Stability: Lyophilized Platelet-derived Growth Factor BB is stable for at least a year. Upon reconstitution, the preparation is stable for up to 10 days at 2-8º C and greater than 3 month at -20º C. Avoid repeated freeze-thaw cycles.
Molecular Mass: 32 KDa.
Biological Activity: Recombinant Human PDGF-BB is fully biologically active when compared to the NIBSC International Standard (IS) for platelet-derived growth factor-BB, which has a specific activity of 3000 I.U/4ug. The ED50, calculated in a dose-dependant proliferation of Swiss mouse 3T3 fibroblasts assay (measured by 3H-thymidine uptake) is 1-3 ng/ml.
Customized sizes upon request.
Usage: For research use only. Not for use in human diagnostics or therapeutic procedures.
Properties
Form
Powder
Research Application
Usage
For research use only. Not for use in diagnostics or therapeutic procedures
Comparison
Synonyms: Platelet Derived rowth Factor-BB, Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin, CYT-BBY000, CYT-BBY, BBY
Other Product Categories: Media, Reagents, Stem Cell Products